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cd45 1 a20 pe cy7  (Cytek Biosciences)


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    Cytek Biosciences cd45 1 a20 pe cy7
    Cd45 1 A20 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+cy7+cd45+1+a20/pmc11393282-57-19-59?v=Cytek+Biosciences
    Average 93 stars, based on 8 article reviews
    cd45 1 a20 pe cy7 - by Bioz Stars, 2026-06
    93/100 stars

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    Single-cell cloning of gene-edited functional HSCs (A) Schematic showing the extracellular domain of CD45 with allele-specific antibody clones 104 and <t>A20</t> and the epitope-defining amino acid. (B) Experimental setup of the single-cell editing and expansion experiment. (C) Left: fractions of CD201 + CD150 + KSL cells in single-cell-derived cultures 14 days after cloning (n = 261 clones). Right: Histogram of CD201 + CD150 + KSL cell frequency. Zoomed-in region shows clones with >10% CD201 + CD150 + KSL cells. (D and E) CD45.1 + donor PB chimerism (D) and lineage distribution (E ) in single recipients with long-term (LT) engraftment ≥5% and multilineage reconstitution (n = 8). Numbers over graphs in (D) represent percentage of CD201 + CD150 + KSL cells in the transplanted clone (%). (F) Linear correlation plots of CD201 + CD150 + KSL cell frequency and 16-week donor chimerism. Red dots indicate LT repopulating and multilineage clones. Pearson correlation. (G) CD45.1 + PB chimerism and lineage distribution in secondary recipients (n = 5). See also <xref ref-type=Figure S4 and Table S3 . Error bars represent SD. " width="250" height="auto" />
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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: G2 arrest primes hematopoietic stem cells for megakaryopoiesis

    doi: 10.1016/j.celrep.2024.114388

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: PE-Cy7 CD45.1 (A20) , Tonbo , Cat# 60-0453-U100; RRID: AB_2621850.

    Techniques: Recombinant, Adhesive, Purification, Blocking Assay, Amplification, Random Hexamer, Reverse Transcription, SYBR Green Assay, Sterility, Imaging, Software

    Single-cell cloning of gene-edited functional HSCs (A) Schematic showing the extracellular domain of CD45 with allele-specific antibody clones 104 and A20 and the epitope-defining amino acid. (B) Experimental setup of the single-cell editing and expansion experiment. (C) Left: fractions of CD201 + CD150 + KSL cells in single-cell-derived cultures 14 days after cloning (n = 261 clones). Right: Histogram of CD201 + CD150 + KSL cell frequency. Zoomed-in region shows clones with >10% CD201 + CD150 + KSL cells. (D and E) CD45.1 + donor PB chimerism (D) and lineage distribution (E ) in single recipients with long-term (LT) engraftment ≥5% and multilineage reconstitution (n = 8). Numbers over graphs in (D) represent percentage of CD201 + CD150 + KSL cells in the transplanted clone (%). (F) Linear correlation plots of CD201 + CD150 + KSL cell frequency and 16-week donor chimerism. Red dots indicate LT repopulating and multilineage clones. Pearson correlation. (G) CD45.1 + PB chimerism and lineage distribution in secondary recipients (n = 5). See also <xref ref-type=Figure S4 and Table S3 . Error bars represent SD. " width="100%" height="100%">

    Journal: Cell Stem Cell

    Article Title: Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion

    doi: 10.1016/j.stem.2023.06.002

    Figure Lengend Snippet: Single-cell cloning of gene-edited functional HSCs (A) Schematic showing the extracellular domain of CD45 with allele-specific antibody clones 104 and A20 and the epitope-defining amino acid. (B) Experimental setup of the single-cell editing and expansion experiment. (C) Left: fractions of CD201 + CD150 + KSL cells in single-cell-derived cultures 14 days after cloning (n = 261 clones). Right: Histogram of CD201 + CD150 + KSL cell frequency. Zoomed-in region shows clones with >10% CD201 + CD150 + KSL cells. (D and E) CD45.1 + donor PB chimerism (D) and lineage distribution (E ) in single recipients with long-term (LT) engraftment ≥5% and multilineage reconstitution (n = 8). Numbers over graphs in (D) represent percentage of CD201 + CD150 + KSL cells in the transplanted clone (%). (F) Linear correlation plots of CD201 + CD150 + KSL cell frequency and 16-week donor chimerism. Red dots indicate LT repopulating and multilineage clones. Pearson correlation. (G) CD45.1 + PB chimerism and lineage distribution in secondary recipients (n = 5). See also Figure S4 and Table S3 . Error bars represent SD.

    Article Snippet: anti-mouse CD45.1-PE/Cy7 (A20) , Tombo Biosciences , Cat#60-0453; RRID: AB_2621850.

    Techniques: Cloning, Functional Assay, Clone Assay, Derivative Assay

    Journal: Cell Stem Cell

    Article Title: Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion

    doi: 10.1016/j.stem.2023.06.002

    Figure Lengend Snippet:

    Article Snippet: anti-mouse CD45.1-PE/Cy7 (A20) , Tombo Biosciences , Cat#60-0453; RRID: AB_2621850.

    Techniques: Recombinant, Sequencing, Software, CRISPR